Journal: Synthetic and Systems Biotechnology

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates

doi: 10.1016/j.synbio.2026.01.006

Figure Lengend Snippet: Utilization of lignocellulosic hydrolysate for succinate fermentation. (A) Preparation process of corn stover hydrolysate including pretreatment, enzymatic hydrolysis, detoxification, and sugar composition analysis; (B–C) Fermentation of ESC6 crp -W68 + in a 5-L bioreactor using synthetic sugar mixtures mimicking hydrolysate composition (B) and actual corn stover hydrolysate (C) as carbon sources. All experimental data were performed in triplicate, and error bars represent the standard deviation.

Article Snippet: For fermentation, the seed culture was inoculated into a 5-L bioreactor (Shanghai Baoxing Bio-Engineering Equipment, Shanghai, China) at a 10 % (v/v) inoculum ratio, with the bioreactor having a working volume of 2 L. During the aerobic growth phase, the air flow rate was maintained at 2 L/min, the agitator speed was controlled between 300 and 550 rpm, the dissolved oxygen level was sustained above 60 %, the temperature was regulated at 37 °C, and the pH was maintained at 6.8 via the addition of 15 % ammonia solution.

Techniques: Standard Deviation

Journal: Synthetic and Systems Biotechnology

Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus

doi: 10.1016/j.synbio.2026.03.002

Figure Lengend Snippet: Fed-batch fermentation of YSAL07 using glucose or xylose mother liquor in a 2-L bioreactor. (A) Glucose as the carbon source. (B) Xylose mother liquor as the carbon source.

Article Snippet: Fed-batch fermentation was conducted in a 2-L bioreactor (Baoxin, Shanghai) with an initial working volume of 1 L at 37 °C.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: Process from bioprinting to in vitro maturation of cartilage-like constructs . (1) The process begins with bioprinting strategies that integrate the three key components of tissue engineering—cell-laden hydrogels, bioactive molecules, and three-dimensional scaffolds—through a multi-material 3D bioprinting process. (2) The resultant construct is subsequently cultured in a bioreactor that provides controlled stimuli to support tissue development and functional maturation, ultimately yielding an engineered cartilage-like construct.

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques: In Vitro, Construct, Cell Culture, Functional Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: (A) TisXell bioreactor with biaxial rotation for 3D cell culture and tissue engineering. The system provides controlled environmental conditions and gentle biaxial motion to enhance nutrient and gas exchange and support mechanotransduction during construct maturation. (B) Schematic representation of the TisXell bioreactor showing the biaxial rotation arm, spin and tumble axes, auxiliary culture bottle, perfusion circuit, and control panel.

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques: Cell Culture, Gentle, Construct, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: (A) Pin-on-ball bioreactor from Wimmer et al.’s work from 2004. (B) Four-station bioreactor capable of applying multiaxial load .

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: A novel bioreactor system for biaxial mechanical loading enhances the properties of tissue-engineered human cartilage . (A) Overview of the bioreactor system. (B) Close-up view of PTFE pistons inserted into a standard 24-well plate. (C) Schematic representation of dynamic biaxial loading applied to hydrogel constructs. (D) Top view of the loading platform and fastening system. (E) Frontal view of the bioreactor chamber and linear bearing system.

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques: Construct

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: (A) Two-DoF IVD bioreactor. Reproduced from . (B) Six-DoF IVD bioreactor by CSEM.

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: Development of a bioreactor for in vitro compression cycling of tissue-engineered meniscal implants. (A) General view of the dock showing the motor mount, support legs, piston tie-bar, and mounted culture chamber secured with removable bumpers and thumbscrews (asterisks indicate the front bumper). (B) Detailed view of the piston tie-bar including the optical mask aperture for reference sensing and the extension used to define the lower travel limit. (C) Side view of the sensor configuration showing the reference sensor and the upper limit sensor that prevents excessive actuator displacement. .

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques: In Vitro

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

doi: 10.3389/fbioe.2026.1717769

Figure Lengend Snippet: (A) Photograph of the BMAP® Knee bioreactor. (B) Photograph of the biocapsule containing culture media, showing the femoral condyles (distal femur) and the tibial plateau (proximal tibia). (C) BMAP® Knee software interface, where parameters such as the average medium temperature, CO 2 levels, pH, and load can be monitored in real-time.

Article Snippet: The Flexcell FX-5000TM Compression System (Flexcell®, Burlington, N.C.) is a computer-regulated bioreactor that applies cyclic or static strain to cells cultured on a pneumatically deformed membrane.

Techniques: Software